Iterative self-assembly

Iterative self assembly is letting things fully self-assemble before mixing in the next kind(s) of building blocks.

• All reactive sites that shall not be filled with one of the parts in the next wash-in should
  be fully saturated before proceeding with the next step.
• There needs to be a way for sufficiently thorough wash-outs and wash-ins such that
  the desired products keep staying in the reaction volume. E.g. attachment to large surfaces provided by some sort of larger scale micro-beads.

Iterative assembly can provide a basis for
circumventing the limitations of de-novo proteins in termination control.

Related

The complement to iterative self-assembly is one-pot self-assembly.
Iterative self-assembly can also be a sequence of one-pot self-assembly steps.