Difference between revisions of "Kinetic trap"

Revision as of 19:08, 5 July 2021

Misassembly trap

In thermally driven self assembly if

• specificity is low but
• bonding strength is high

things may self assembly in a wrong way and stay there for good.

If the bonding strength is deliberately kept low then even after the fact of a faulty self-assembly-bonding-event
by thermal vibrations the formed bond can be jostled apart again
and the part gets a another chance for assembling into one of the intended binding spots.

In first approximation that typically works the better the slower the cooling profile during selfassembly (thermal annealing).

Assembly path cutoff trap

Even without any faulty self-assembly-bonding-events a kinetic trap can emerge.
If the order of assembly is by chance such that access to yet unfinished parts gets and irreversibly stays blocked, that part can never be finished.

Example:
DNA bricks in structural DNA nanotachnology may assemble such that some voxels that should be filled with an DNA oglionucleotide strands
just remain unfilled since the structure around was built up before they got a chance to be filled.

The degree to which this happens may be hard to tell since the imaging technology cryo-TEM-tomography
for the highest resolution images needs to use averages over a large number of images of assemblies to get the fimal pictures (lots of fourier math involved).